Reporter

Part:BBa_K187018:Design

Designed by: Team BioBytes   Group: iGEM09_Alberta   (2009-10-17)


GFP in pAB, BioBytes plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 10
    Illegal PstI site found at 736
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 736
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 10
    Illegal PstI site found at 736
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 10
    Illegal PstI site found at 736
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 659


Design Notes

This plasmid includes only the open reading frame (start codon to stop codon) of GFP. A promoter and terminator are not included. pAB and pBA do include an RBS consensus sequence positioned 8 bp upstream of the ATG. We recommend that to express GFP, you use the Biobytes assembly method together with the promoter and terminator parts we have submited in pAB and pBA to assemble promoters and terminators onto GFP. As there is a range of promoters to chose from, this allows rapid manipulation of gene expression level. Using the Biobytes method, several DNA segments can be combined in just 20min per segment.


Source

Part BBa_J61003 was the source of GFP. Biobytes plasmid pAB is entered as BBa_K187000.

References